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. 2003 Sep;77(18):9894–9905. doi: 10.1128/JVI.77.18.9894-9905.2003

TABLE 1.

Pre- and postchallenge passive transfer of sera from rhesus macaques vaccinated with pWRG/AND-M to Syrian hamsters

Hamster no. Antibody
Day 0 PRNT titerc Challenge virusd Day of deathe Terminal bleedf
% Protection
Sourcea Dayb injected PRNT titerg ELISA titerh From lethality From infectioni
905 AND-M −1 320 ANDV − <20 <100 50 50
906 −1 640 ANDV − <20 <100
907 −1 320 ANDV − <20 <100
908 −1 1,280 ANDV − <20 <100
105 −1 20 ANDV 23 NA NA
106 −1 40 ANDV 23 NA NA
107 −1 20 ANDV 40 NA NA
108 −1 20 ANDV 40 NA NA
901 Negative control −1 <20 ANDV 12 NA NA 0 0
902 −1 <20 ANDV 13 NA NA
903 −1 <20 ANDV 10 NA NA
904 −1 <20 ANDV 13 NA NA
101 −1 <20 ANDV 10 NA NA
102 −1 <20 ANDV 14 NA NA
103 −1 <20 ANDV 10 NA NA
104 −1 <20 ANDV 11 NA NA
909 AND-M 3 <20 ANDV − <20 <100 88 14
910 3 <20 ANDV − <20 400
911 3 <20 ANDV − 2,560 12,800
912 3 <20 ANDV − 160 800
109 3 <20 ANDV − >640 1,600
110 3 <20 ANDV − 640 6,400
111 3 <20 ANDV 12 NA NA
112 3 <20 ANDV − >640 3,200
113 AND-M 4 <20 ANDV − 160 400 100 0
114 4 <20 ANDV − 640 400
115 4 <20 ANDV − >640 12,800
116 4 <20 ANDV − 320 1,600
117 AND-M 5 <20 ANDV − 320 1,600 100 0
118 5 <20 ANDV − >640 1,600
119 5 <20 ANDV − 640 800
120 5 <20 ANDV − 160 400
121 AND-M 6 <20 ANDV 11 NA NA 38 0
122 6 <20 ANDV − 40 800
123 6 <20 ANDV 13 NA NA
124 6 <20 ANDV 12 NA NA
913 6 <20 ANDV 11 NA NA
914 6 <20 ANDV − 1,280 12,800
915 6 <20 ANDV 10 NA NA
916 6 <20 ANDV − 1,280 6,400
917 AND-M 9 <20 ANDV 11 NA NA 25 0
918 9 <20 ANDV 13 NA NA
919 9 <20 ANDV − 10,240 12,800
920 9 <20 ANDV 11 NA NA
921 AND-M −1 160 PBS − <20 <100 100 100
922 −1 640 PBS − <20 <100
923 −1 <20 PBS − <20 <100
924 −1 160 PBS − <20 <100
a

Serum from monkey vaccinated with pWRG/AND-M (AND-M) or negative control sera. The monkey serum used in experiment 1 (no. 909 to 924) had a PRNT50 titer of 20,480, and the serum used in experiment 2 (no. 109 to 124) had a PRNT50 titer of 1,280.

b

Day antibody injected relative to challenge (day 0).

c

NAb titer in hamster serum at time of challenge (PRNT50).

d

2,000 PFU of ANDV (250 LD50) or PBS was injected i.m. on day 0.

e

−, animal survived.

f

In experiments 1 and 2, the terminal bleed was obtained on days 28 and 98, respectively.

g

Reciprocal of highest dilution neutralizing 50% of the plaques; NA, not applicable.

h

Endpoint ELISA titer. NA, not applicable.

i

Protection from infection (sterile immunity) indicates no detectable antibody response to nucleocapsid in terminal bleed.