Table II.

A Pro-35S:AtPAP15 mutant has higher phytase activity than wild-type plants, whereas a knockout mutant (Atpap15) has lower phytase activity

Plant protein extracts were partial purified on a concanavalin A column. Phytase activity was measured as released Pi (A₃₅₅). Values represent mean (n = 3).

Substrate	Enzyme Activity
		Wild Type	Pro-35S:AtPAP15	Atpap15
	nmol Pi min−1 mg protein−1
pNPP	925 ± 56	1,577 ± 100	976 ± 101
Phytate	68 ± 3	129 ± 21	12 ± 8
NaPP	1,760 ± 691	3,219 ± 206	2,058 ± 363