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. 2007 Dec 19;8:467. doi: 10.1186/1471-2164-8-467

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Copyright © 2007 Ulvé et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Identification of small "known" RNAs in the S. meliloti chromosome. A: Migration of 10 μg of S. meliloti total RNA in a 2.5% high-resolution agarose gel (Sigma) stained with ethidium bromide. B: 5 μg of total RNA from S. meliloti strain 1021 was analyzed by Northern blotting hybridization with specific oligonucleotides [see Additional file 8]; the molecular sizes were calculated in nucleotides (nt): > rnpB (372 nt), > tmRNA 3' end (204 nt), > 5S (120 nt), > 4.5S (95 nt), and > tmRNA 5' end (82 nt) [39]. Band > was not hybridized but its size is consistent with it being a tRNA. Exposure times were optimized for each panel and signal intensity does not indicate relative abundance of ncRNAs.

Inline graphic — Identification of small "known" RNAs in the S. meliloti chromosome. A: Migration of 10 μg of S. meliloti total RNA in a 2.5% high-resolution agarose gel (Sigma) stained with ethidium bromide. B: 5 μg of total RNA from S. meliloti strain 1021 was analyzed by Northern blotting hybridization with specific oligonucleotides [see Additional file 8]; the molecular sizes were calculated in nucleotides (nt): > rnpB (372 nt), > tmRNA 3' end (204 nt), > 5S (120 nt), > 4.5S (95 nt), and > tmRNA 5' end (82 nt) [39]. Band > was not hybridized but its size is consistent with it being a tRNA. Exposure times were optimized for each panel and signal intensity does not indicate relative abundance of ncRNAs.