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. Author manuscript; available in PMC: 2008 Feb 15.
Published in final edited form as: Cancer Cell. 2008 Jan;13(1):23–35. doi: 10.1016/j.ccr.2007.12.004

Figure 2. Gr-1+CD11b+ cells promote tumor metastasis.

Figure 2

A: Increased Gr-1+CD11b+ cells in spleens and bone marrow of 4T1 tumor-bearing mice after tumor inoculation. Left panel: Flow cytometry analysis of Gr-1+CD11b+ cells in the spleen of tumor bearing mice 28 days following engraftment (4 or more mice per time point). Middle panel: spleen; Right panel: bone marrow (b.m.). B: Gr-1+CD11b+ cells in 4T1 tumors with flow cytometry analysis. C: IHC showing Gr-1+CD11b+ cells in the invasive front of 4T1 tumors 15 day after tumor inoculation. Scale bar, 100 μM. D: Single cell sorting of Gr-1+CD11b+ cells from tumors and spleens of 4T1 tumor-bearing mice 35 days after tumor inoculation. Flow cytometry analysis of Gr-1+CD11b+ cells after sorting is shown. E: Metastasis was significantly increased when 4T1 cells were co-injected with tumor derived Gr-1+CD11b+ cells including Gr-1+CD11b+ cells derived from tumor tissues (tu inf Gr-1+CD11b+ cells) spleens of tumor-bearing mice (tu sp Gr-1+CD11b+ cells). 4T1 cells alone and Gr-1+CD11b+ cells from normal mice (nor Gr-1+CD11b+ cells) were used as controls. Number of animals used is indicated in the bar. m.g: adjacent mammary gland tissue; tu: tumor tissues. Results are presented as the mean ± SE.