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. 2003 Sep;77(18):9987–9992. doi: 10.1128/JVI.77.18.9987-9992.2003

FIG. 3.

FIG. 3.

Effect of Nedd4 mutants on Ebola VLP production. (A) VLPs produced from cells coexpressing VP40 and each Nedd4 mutant were analyzed as in Fig. 1A (upper panel). The expression of VP40 (middle panel) and Nedd4 mutants (lower panel) were also examined. AAXY indicates the VLP sample from cells cotransfected with pCEboZVPAAXY and a control vector. The position of VP40 is indicated on the right. Asterisks on the left indicate the migration position of the putative Nedd4 or Nedd4 mutant proteins incorporated into VLPs in the upper panel and indicate the actual position of Nedd4 or Nedd4 mutants in the lower panel. (B) The intensity of the band for VLP-associated VP40 in panel A was quantified as described in Materials and Methods. The extent of VP40 in the VLPs released from cells cotransfected with pCEboZVP40 and a control vector was set to 100%. The data represent averages and SD of three independent experiments.