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. 2003 Sep;77(18):9987–9992. doi: 10.1128/JVI.77.18.9987-9992.2003

FIG. 4.

FIG. 4.

Effect of overexpression of Tsg101 or its mutant on Ebola VP40-induced VLP budding. (A) Extracellular VLPs from cells cotransfected with pCEboZVP40 and either the expression plasmid for Tsg101 or the expression plasmid for TsgΔC were analyzed as in Fig. 1A (upper panel). The expression levels of VP40 (middle panel) and Tsg101 or TsgΔC (lower panel) were also determined. The position of VP40 is indicated on the right. The asterisk on the left indicates the protein detected only in VLPs from cells expressing Tsg101. (B) The intensity of the band for VLP-associated VP40 in panel A was quantified as described in Materials and Methods. The extent of VP40 in the VLPs released from cells cotransfected with pCEboZVP40 and a control vector was set to 100%. The data represent averages and SD of three independent experiments.