Combining site structure of mAb 735 and structural models of 2-2-B and anti-N-Pr MBPS mAbs SEAM 2, SEAM 3, SEAM 12, SEAM 18 and SEAM 35. The structures are shown as surface renderings and are arranged according to relative autoantibody activity from mAb 735 clockwise to SEAM 3 and SEAM 2, which have no autoantibody activity. The surface is colored according to electrostatic surface potential charge with dark blue corresponding to a charge of +5 and dark red a charge of −1. The surface potentials were calculated using APBS operating in Pymol. Note the dark shading between the light chain above and heavy chain below for the SEAM 2 and SEAM 3 models showing the presence of a deep cleft and pocket-like features, respectively.