Table 2.
Comparison of the genetic origin of variable regions of anti-MBPS and anti-N-Pr MBPS mAbsa
| Clone | Isotype | VH | DH | JH | VL | JK |
|---|---|---|---|---|---|---|
| 735 | IgG2a,κ | IGHV653*01 | IGHD-Q52*02 | IGHJ2*01 | IGKV1-110*02 | IGKJ5*01 |
| 2-2-B | IgM,κ | J558.2 | IGHD-SP2.7*01 | IGHJ2*01 | IGKV1-110*01 | IGKJ2*01 |
| SEAM 2 | IgG3,κ | IGHV1S4*01 | NDb | IGHJ2*01 | IGKV1-135*01 | IGKJ5*01 |
| SEAM 3 | IgG2b,κ | IGHV7S3*02 | IGHD-SP2.8*01/inv | IGHJ2*01 | IGKV1-135*01 | IGKJ5*01 |
| SEAM 12 | IgG2a,κ | IGHV13S1*01 | IGHD-ST4*01 | IGHJ2*01 | IGKV4-63*01 | IGKJ5*01 |
| SEAM 18 | IgG2b,κ | IGHV7S3*02 | IGHD-SP2.8*01/inv | IGHJ2*01 | IGKV4-74*01 | IGKJ2*01 |
| SEAM 35c | IgG2a,κ | IGHV7S3*02 | NDb | IGHJ4*01 | IGKV6-20*01 | IGKJ2*01 |
Closest matches from either the IGMT or GenBank/EMBL databases. MAb 735 is from a hybridoma produced from a NZB mouse immunized with viable N. meningitidis group B strain ATCC 13090 (Frosch et al., 1985). Since only the amino acid sequences were reported (Klebert et al., 1993; Vaesen et al., 1991), assignment of the germline genes for this mAb was based on the closest match (see Section 2). MAb 2-2-B was produced from a BALB/CJ mouse immunized with N. meningitidis group B strain P355. Germ line assignments were based on the published DNA sequences (Berry et al., 2005). The SEAM mAbs are from hybridomas prepared from CD1 mice immunized with N-Pr MBPS tetanus toxoid conjugate (Granoff et al., 1998).
ND, not determined since the segment was too short to enable identification of a specific gene.
Note that in our original paper this mAb was listed as being IgG2b, we have subsequently confirmed that it is lgG2a. The subclasses of all of the other anti-N-Pr MBPS mAbs used in this study were retested and confirmed as listed here and in (Granoff et al., 1998).