Fig. 5.

Two larger and one intermediate-size plaque gap junction in P14 rat SCN double-labeled for Cx32 (none present) and Cx36. (A–B) Single intermediate-size plaque gap junction (65 connexons) labeled for Cx36 by nine 6-nm gold beads (arrows) and two 18-nm gold beads. (C) Slightly larger neuronal gap junction with 123 connexons labeled by seven 6-nm gold beads (arrows) and one 18-nm gold bead. (D) Neuronal gap junction with ca. 199 connexons labeled by four 6-nm gold beads (arrows) and two 18-nm gold beads. Because of the low local angle of shadow, more than half the connexons were not shadowed with platinum but are faintly delineated by the primary carbon coat, as discerned by stereoscopic viewing (C–D). The edge of gap junction is indicated by arrowheads. The number of connexons was calculated based on area of gap junction and 10-nm spacing in plaque gap junctions (Kamasawa et al., 2006).