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. Author manuscript; available in PMC: 2008 Feb 15.
Published in final edited form as: Neuroscience. 2007 Jul 21;149(2):350–371. doi: 10.1016/j.neuroscience.2007.06.052

Table 1.

Antibodies and concentrations used for immunohistochemistry and FRIL.

Antibody Type Species Epitope; Designation Dilution* Source
Cx36 polyclonal rabbit c-terminus; Ab36-4600 1 μg/ml, 10 μg/ml Invitrogen/Zymed
Cx36 polyclonal rabbit c-terminus; Ab51-6300 1 μg/ml, 10 μg/ml Invitrogen/Zymed
Cx36 polyclonal rabbit aa298-318; Rash et al., 2000 10 μg/ml JI Nagy**
Cx36 monoclonal mouse c-terminus; Ab37-4600 3 μg/ml, 10 μg/ml Invitrogen/Zymed
Cx32 polyclonal rabbit c-terminus; Ab34-5700 1 μg/ml Invitrogen/Zymed
Cx32 polyclonal rabbit cytoplasmic loop; Ab71-0600 10 μg/ml Invitrogen/Zymed
Cx32 monoclonal mouse cytoplasmic loop; Ab13-8200 10 μg/ml Invitrogen/Zymed
Cx32 monoclonal mouse aa95-125, mid region; MAB3069 10 μg/ml Chemicon
Cx30 polyclonal rabbit c-terminus; Ab71-2200 3 μg/ml, 10 μg/ml Invitrogen/Zymed
Cx43 monoclonal mouse c-terminus; Ab35-5000 3 μg/ml Invitrogen/Zymed
Cx43 monoclonal mouse c-terminus; MAB3068 10 μg/ml Chemicon
AQP4 polyclonal rabbit c-terminus; AB3068 10 μg/ml Chemicon
CNPase monoclonal mouse whole protein 1:2000 Sternberger Monoclonals
*

For FRIL, all antibodies were used at 10 μg/ml.

**

Previously characterized in Rash et al. (2000)

aa, amino acids