Effective repression of eme by Lim3 requires the M1b, M1c and Lb2 sites, but not the M2a site. (A,B) eme with M1b (A) or M2a (B) site mutated. (C–F) Overexpression of lim3 in embryos which carry eme reporter with M1b site (C), Lb2 site (D), M1c site (E) or M2a site (F) mutated. Note the lack of repression of the reporter in the absence of a M1b, M1c or Lbe site and almost complete repression of reporter in the absence of M2a site. (G,H) Mesodermal msh overexpression strongly represses Eve and wildtype eme reporter expression (G) but not Lb2* mutant eme-lacZ expression (H).