FIGURE 10.
HLA-A2-MART-127–35 complex expression on 1074MEL.β2.7 melanoma cells. The neo (1074MEL.neo)- and β2m-transfected (1074MEL.β2.7) 1074MEL cells, the neo (1259MEL.neo)- and β2m-transfected (1259MEL.β2.18) 1259MEL cells, and the β2m-transfected 1106MEL cells (1106MEL.β2) were stained with the HLA-A2-MART-127–35 complex-specific scFv 8.3 tetramer (scFv 8.3 tet) and analyzed by flow cytometry. The HLA-A2, -A28-specific mAb KS-1, the HLA-A2+MART-1+ melanoma cells 501, and the HLA-A2+MART-1− lymphoblastoid cells LG-2 were used as controls.