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Selective HLA-A2 Ag loss by β2m-transfected 1259MEL melanoma cells. Control- and IFN-γ-treated (300 U/ml, 48 h at 37°C) β2m-transfected 1074MEL (1074MEL.β2.7) and β2m-transfected 1259MEL (1259MEL.β2.18) cells were stained with the mAb KS-1 and CR11–351 and analyzed by flow cytometry. The mAb Q5/13 was used to assess HLA class II Ag expression following treatment of these melanoma cells with IFN-γ. Results of one of three representative experiments are shown.
