FIG. 7.
IL-18 production in response to VV infection in vivo and in vitro. (A) IL-18 levels in BAL. Mice were infected with 104 PFU of vC12L, vΔC12L, or vC12L-rev and were sacrificed 3 or 7 days later. BALs from four to five mice were pooled, and the level of total IL-18 protein was determined by ELISA. Data are expressed as the mean ± standard error from two independent experiments. (B) IL-18 release by lung macrophages. Lung macrophages were isolated from naive BALB/c mice and cultured in the presence of 10 PFU of vC12L, vΔC12L, or C12L-rev per cell. Supernatants were measured for IL-18 by ELISA. Results are expressed as the mean ± standard error of duplicate wells.