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Guanosine treatment did not affect apoptosis in the cords with chronic, stable spinal cord injury. For detection of apoptotic cells, a terminal deoxynucleotidyltransferase (TdT)-mediated dUTP nick end labelling (TUNEL) stain was performed using the ‘In situ Cell Death Detection Kit, Fluorescein’. No obvious difference in number of TUNEL-positive cells was observed between vehicle- (a) or guanosine- (b) treated spinal cord sections. Scale bar = 50 μm for all
