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. Author manuscript; available in PMC: 2008 Feb 28.
Published in final edited form as: Mol Endocrinol. 2006 Mar 9;20(7):1506–1518. doi: 10.1210/me.2005-0286

Figure 3. RIP140 inhibits AR-dependent transactivation.

Figure 3

A. RIP140 represses AR-dependent transactivation. CV1 cells were transfected using the calcium phosphate method with 10 ng of pCMV-AR and increasing amounts of pcRIP140. The pcDNA empty vector was used to keep the amount of DNA transfected constant.

B. AR is overactivated in cells devoid of RIP140. Wild type (wt) or RIPKO (−/−) MEF cells were cotransfected with either 10 ng or 50 ng of pCMV-AR using the calcium phosphate method.

C. Different domains of RIP140 participate in AR-dependent transactivation. 10 ng of pCMV-AR as well as 800 ng of each mutant were transfected in CV1 cells using the calcium phosphate method.

D. RIP140 reverses TIF2-induced AR activity. CV1 cells were transfected with 10 ng of pCMV-AR, 200 ng of psg5-TIF2 and increasing amounts of pcRIP(917-1158).

The luciferase activity was expressed taking AR in the presence of R1881 as 100 % of the activity. The mean ± SD values from at least three independent experiments are shown.

■ = Ethanol; Inline graphic = R1881 (10−9 M).