A. RIP140 has no effect on AR(1-660)-dependent transactivation. CV1 cells were transfected as above with 100 ng of pCMV-AR(1-660) and increasing amounts of pcRIP140. The luciferase value given by pCMV-AR(1-660) in the absence of pcRIP140 was determined as 100 %.
B. RIP140 inhibits AR(507-919)-dependent activity. CHO cells were transfected using the FUGENE-6 according to the manufacturer’s instructions with 100 ng of pCMV-AR(507-919), with or without psg5-TIF2 and pEF-RIP140. The luciferase activity was expressed taking AR(507-919) + TIF2 as 100 % of the activity.
The mean ± SD values from at least three independent experiments are shown.