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. Author manuscript; available in PMC: 2008 Feb 28.
Published in final edited form as: Mol Endocrinol. 2006 Mar 9;20(7):1506–1518. doi: 10.1210/me.2005-0286

Figure 4. RIP140 exerts its repression via the carboxy-terminal domain of AR.

Figure 4

A. RIP140 has no effect on AR(1-660)-dependent transactivation. CV1 cells were transfected as above with 100 ng of pCMV-AR(1-660) and increasing amounts of pcRIP140. The luciferase value given by pCMV-AR(1-660) in the absence of pcRIP140 was determined as 100 %.

B. RIP140 inhibits AR(507-919)-dependent activity. CHO cells were transfected using the FUGENE-6 according to the manufacturer’s instructions with 100 ng of pCMV-AR(507-919), with or without psg5-TIF2 and pEF-RIP140. The luciferase activity was expressed taking AR(507-919) + TIF2 as 100 % of the activity.

The mean ± SD values from at least three independent experiments are shown.