Table II. Effects of ceramides on the release of intermembrane space proteins.

Individual bands from polyacrylamide gels such as the one illustrated in Fig. 4 were quantitated by densitometry. The intensity was corrected for gel background and for the intensity of the corresponding band in the untreated mitochondrial control. The results were expressed as a percentage of the intensity of the corresponding band for protein released after selective damage of the outer membrane by hypotonic shock. Errors represent the S.E. of the mean for three separate mitochondrial preparations.

Band	Release as % of intermembrane space content
	C16-ceramide	C2-ceramide
kDa
55.7 ± 0.5	15.3 ± 1.5	12.7 ± 1.8
44.4 ± 0.9	15.1 ± 2.3	14.7 ± 2.7
36.3 ± 1.5	13.0 ± 2.7	12.6 ± 2.2
22.6 ± 0.4	14.1 ± 1.5	12.8 ± 1.7
18.7 ± 0.2	16.1 ± 1.2	14.9 ± 1.3
12.6 ± 0.3	12.4 ± 1.8	12.3 ± 2.0
10.5 ± 0.3	16.7 ± 2.1	15.9 ± 2.6