Proportion of PAS template in plants harvested 33 days post primary inoculation with PAS. Inoculation treatments were: equal amounts of leaf tissue from plants singly infected with PAV and PAS paired prior to nucleic acid extraction (one:one), simultaneous inoculation with PAV and PAS, PAS challenged with PAV three days later (PAS 3 day) and PAS challenged with PAV 15 days later (PAS 15 day). Box plots show the median, 5th, 25th, 75th and 95th percentiles.