Table 1.

The best-fit line when the relative amount of PAV template in the standard sample was regressed onto PAV as a proportion of the total peak area or height [PAV / (PAV + PAS)] at five nucleotide sites that differentiate PAV and PAS.

Sitea	templateb	peak measurement	Function
2074		area	- 0.097 + 15.95 area - 6.73 area2
	RNA	height	0.13 + 25.53 height - 31.9 height2 + 15.5 height3
		area	- 0.006 + 15.24 area - 5.75 area2
	DNA	height	- 0.27 + 23.6 height - 29.3 height2 + 16.22 height3
2080		area	- 0.33 + 9.63 area
	RNA	height	- 0.021 + 9.6 height
		area	- 0.46 + 15.26 area - 5.96 area2
	DNA	height	- 1.14 + 14 height - 3.23 height2
2083		area	1.6 + 8.05 area
	RNA	height	0.49 + 9.25 height
		area	- 0.19 + 9.049 area
	DNA	height	- 0.82 + 10.83 height
2086		area	5.92 - 20.92 area + 23.96 area2
	RNA	height	9.34 height 2.136
		area	- 1.45 + 19.45 height - 46.86 area2 + 37.7 area3
	DNA	height	3.96 - 17.24 height + 22.06 height2
2088		area	1.95 + 11 area − 3.6 area2
	RNA	height	- 3.1 + 37.9 height - 47.08 height2 + 22.12 height3
		area	1.95 + 11 area - 3.6 area2
	DNA	height	- 0.12 + 41.8 height - 77.32 height2 + 47 height3

^acomplete genome of PAV-Aus (M21347) used as the reference sequence;

^bstandard curves were created by mixing in vitro transcribed viral RNA or RT-PCR products in ratios from (1:9 to 9:1 PAV:PAS).