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. 2003 Sep;77(18):10004–10014. doi: 10.1128/JVI.77.18.10004-10014.2003

FIG. 1.

FIG. 1.

WNV genome and replicons with or without reporters constructed in this study. Compared with full-length WNV genome, the original replicon (Rep) contained an in-frame deletion of the structural region (dotted open box) from nt 190 to 2379. The N-terminal 31 amino acids from the capsid protein and the C-terminal 30 amino acids from the envelope protein were retained to maintain the essential 5′CS element and to ensure correct processing of the polyprotein, respectively. For construction of reporter replicons, Rluc driven by an IRES was inserted into the upstream end of the 3′ UTR of the Rep in either plus- or minus-sense orientation, resulting in (+)3′RlucRep and (−)3′GFPRep, respectively. Alternatively, Rluc reporter was fused in-frame with the ORF of the Rep in the position where the structural region was deleted, resulting in RlucRep. RlucRep-NS5mt contains a single-nucleotide frameshift upstream of the active site of the RdRp domain of NS5. The nucleotide positions were numbered according to the sequence of WNV strain 3356 (GenBank accession number AF404756). The drawing is not to scale.