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. 2003 Sep;77(18):10004–10014. doi: 10.1128/JVI.77.18.10004-10014.2003

FIG. 2.

FIG. 2.

An Rluc reporter can be used to monitor viral RNA replication. (A) Rluc activities of cell lysates prepared at 34, 48, and 72 h after transfection with RlucRep or RlucRep-NS5mt; (B) IFA of BHK cells transfected with RlucRep or RlucRep-NS5mt at the indicated time points; (C) real-time RT-PCR quantification of RlucRep or RlucRep-NS5mt. RNA was quantified by reference to RNA extracted from a virus stock of a known titer (measured in PFU). Results of one of two representative experiments are shown.