Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
RNA stability of WT and mutant replicons in BHK cells. Equal amounts of WT RlucRep, Rluc-CS1*, Rluc-CS2-del, and Rluc-CS3-del were transfected into BHK cells as described in Materials and Methods. Replicon RNAs were extracted and quantified by real-time RT-PCT at 3, 5, 8, or 12 h posttransfection. The amount of RNA at each time point was estimated by reference to in vitro-transcribed RNA in copy number.
