Fig. 4.

Sox1 heterozygosity partially rescues the size of Pax6^Sey/+ lenses. Representative lenses at E13.5 are shown for wild-type (A); Sox1^+/− (B); Sox1^−/− (C); Pax6^Sey/+ (D–D’); Pax6^Sey/+, Sox1^+/− (E–E’); and Pax6^Sey/+, Sox1^−/− (F–F’) lenses. High magnification images (D’–F’) show epithelial layer abnormalities. Yellow arrows indicate fiber cell nuclei that have remained in the lens posterior (C, F). White arrows indicate multi-layered AEL (F’) and blue arrows indicate pointed AEL (D’, F’). (G) Maximum lens diameters for wild-type (white box), Pax6^Sey/+ (black box), and Pax6^Sey/+, Sox1^+/− (gray box) lenses are statistically different (see Experimental Procedures).