Fig. 5.

Sox1 heterozygosity increases the number of fiber cells in Pax6^Sey/+ lenses. E-cadherin immunofluorescence (red) and DAPI nuclear stain (blue) are shown (A–D) for wild-type (A), Sox1^+/− (B), Pax6^Sey/+ (C) and Pax6^Sey/+, Sox1^+/− (D) lenses at E13.5. (E) E-cadherin negative, fiber cell nuclei were counted for wild-type (white box), Sox1^+/− (light grey box), Pax6^Sey/+ (black box) and Pax6^Sey/+, Sox1^+/− (dark grey box) lenses and the number of fiber cell nuclei in Pax6^Sey/+, Sox1^+/− lenses is statistically significantly higher than those in Pax6^Sey/+ lenses (see Experimental Procedures).