Skip to main content
. 2003 Sep;77(18):10162–10167. doi: 10.1128/JVI.77.18.10162-10167.2003

FIG. 2.

FIG. 2.

Detection of WSSV ORF 151, 366, and 427 transcripts in SPF shrimps by nested PCR. (a and b) The up-regulated genes in the array of SPF shrimps were validated by nested PCR using total cDNA (a) and DNA (b) from SPF shrimps. WSSV-infected RNA and DNA were also included as a positive control. Lanes 1, WSSV ORF 151 (510 bp); lanes 2, WSSV ORF 427 (900 bp); lanes 3, WSSV ORF 366 (210 bp); lanes 4, β-actin transcript (339 bp). (c) Two other genes, VP24 (266 bp) (A) and WSSV ORF 249 (726 bp) (B) were detected via nested PCR in WSSV-infected DNA (lane 1), WSSV-infected RNA (lane 2), and SPF shrimp DNA (lane 3), but not in SPF shrimp RNA (lane 4). Results are representative of all four groups of commercial SPF shrimps.