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Amplification profiles (a) and dissociation curves (b) of WSSV ORF 151 using total RNA from WSSV-infected shrimps and SPF shrimps and aRNA from SPF shrimps. The crossing points are 6.89, >11, and 3.99, respectively. The negative control is a SYBR Green RT-PCR mix without template. The thermal denaturation values of amplicons are indicated alongside the corresponding dissociation curves.
