TABLE 1.
Primer sequences used in the two-step conventional PCR and SYBR Green real-time RT-PCR
| Primer | WSSV ORF | Forward sequence | Reverse sequence |
|---|---|---|---|
| PCR outer primers | 151 | ATGGATTTTGAAGGAACTACCA | CTTCTTTGTTTTCTTTG |
| 366 | ATGAGGAAAATGACCTCTATGA | TCAAGAAAGCGCGTGCTTTAG | |
| 427 | ATGGCATGGACCGTAATGGC | TTCCTTGATCTAGAGCT | |
| PCR inner primers | 151 | GTGGTCACATCTGACAGTGGA | GCATAATGCAGTAGCGTCAACGGC |
| 366 | GAGACGTCGCTCATCAAAGATGGGGAAG | GAAACCTGGACCATATTGAATACGGCCAG | |
| 427 | GAGCTGGCAAAGGAAACC | ACAGACAACAGAACCTCCTTC | |
| Real-time RT-PCR primers | 151 | GGTCGAATCTGACAGGCGTCTTT | CTCCACAACTGCGTTACCTTCGTAT |
| 366 | TGAGAGTGATGATGACGACGATGA | TGCTTTAGCCGGACGGTTAAGAA | |
| 427 | ATAATAATAGTGCCCACCAAATGTCGG | GTTGTGACTGGAGTGTTGATTGGGTA |