TABLE 1.
Construct | Amt of core protein (ng/million cells)
|
|
---|---|---|
Intracellulara | Extracellularb | |
β-Gal | Negativec | Negative |
Dj | 112 | 1.9 |
Dj-C191 | 125 | 1.0 |
Dj-C173 | 23 | 1.5 |
Dj-D111A | 72 | 2.5 |
Cell lysates were tested at a dilution of 1:1,000, and overflow samples were then tested after 1:5,000 dilution.
Cell culture supernatants were tested undiluted, and overflow samples were then tested after 1:10 dilution (all dilutions were made with fresh culture medium).
Below the cutoff value (2.5 pg/ml).