Table 5.
Parameters of individual and composite linkage maps of white spruce and black spruce.
| Mapping parameters | Cross/Parents for white spruce (Picea glauca) | Cross/Parents for black spruce (species complex Picea mariana × P. rubens) | ||||
| Parents | Composite | Parents | Composite | |||
| ♀80112 | ♂80109 | ♀11307-03 [♀83 × ♂425] | ♂425 | |||
| Total number of available markers | 525 | 597 | 1039 | 587 | 563 | 1260 |
| Number of distorted markersb | 7 | 11 | 17 | 12 | 3 | 28 |
| Total number of markers without segregation distortion | 518 | 586 | 1022 | 575 | 560 | 1232 |
| Total number of assigned markers | 509 | 581 | 957 | 534 | 542 | 1064 |
| Number of AFLP loci | 256 | 299 | 581 | 247 | 242 | 679 |
| Number of RAPD loci | 0 | 0 | 0 | 2 | 1 | 3 |
| Number of SSR loci | 9 | 10 | 13 | 27 | 27 | 45 |
| Number of ESTP gene loci | 22 | 23 | 35 | 26 | 30 | 34 |
| Number of SNP gene loci | 222 | 249 | 328 | 232 | 242 | 303 |
| Number of positioned markers (%) | 483 (91.0) | 523 (90.0) | 821 (85.8) | 461 (86.3) | 479 (88.4) | 835 (78.5) |
| Number of AFLP loci | 242 | 257 | 461 | 185 | 188 | 469 |
| Number of RAPD loci | 0 | 0 | 0 | 2 | 1 | 2 |
| Number of SSR loci | 8 | 9 | 12 | 25 | 27 | 36 |
| Number of ESTP gene loci | 19 | 19 | 31 | 23 | 27 | 30 |
| Number of SNP gene loci | 214 | 238 | 317 | 226 | 236 | 298 |
| Number of positioned accessory markers | 4 | 3 | 9 | 0 | 6 | 22 |
| Number of major linkage groups (nb of sub-groups) (n > 10 markers) | 12 (4c) | 12 (1c) | 12 | 12 (2c) | 12 (2c) | 12 |
| Number of minor linkage groups (3 ≤ n ≤ 10 markers) | 0 | 1 | 0 | 0 | 0 | 0 |
| Number of unlinked markers | 9 | 5 | 65 | 41 | 18 | 168 |
| Total observed map length GF, cM (Kosambi) | 2146.1 | 2283.6 | 2304.2 | 1833.5 | 1814.1 | 1849.8 |
| Average map density, cM (Kosambi) | 4.4 | 4.4 | 2.8 | 4.0 | 3.8 | 2.2 |
| Average size for major linkage groups, cM (Kosambi) | 134.1 | 163.1 | 192.1 | 130.9 | 129.5 | 154.1 |
| Expected map length Ge, cM (Kosambi) | 3204.5 | 3569.9 | -d | 4009.4 | 3424.7 | -d |
| Map coverage Ce (%) | 98.3 | 98.4 | -d | 97.7 | 98.1 | -d |
aFor individual linkage maps, only markers segregating 1:1 or 1:1:1:1 were used.
bBonferroni correction: P ≤ 0.01/number of loci.
cNumber of linkage group composed of 2 sub-groups having more than 10 markers.
dCould not be calculated due to the merging of data.