TABLE 1.
Sequences of the oligonucleotides used in these studies
| Gene | Fragment | Annealing temp (°C) | MgCl2 (mM) | Oligonucleotide | Sequence (5′→3′)a |
|---|---|---|---|---|---|
| crmB | 1 | 50 | 2 | CKBP1 | CCCAAGCTTACCATTACAAATATTATTCATAATAG |
| CKBP8 | CGCGGATCCAACATCGATTCCCATATGATGG | ||||
| crmC | 1 | 50 | 2 | CrmC2 | GTGATATACAGCTATTAATTTCG |
| CrmC4 | GGCATTTTCTACATTGATGACATCC | ||||
| 2 | 50 | 2 | CrmC3 | CACAAAATGTGAACGCTGCCTACC | |
| CrmC5 | GTAATTATTAATATGTCTTCATACTG | ||||
| crmD | 1 | 50 | 2 | CrmD1 | CGAGATAATATCCAAGTTAAGCG |
| CrmD6 | TCTGTCCTGTGTGTTACTACACG | ||||
| 2 | 50 | 2 | CrmD3 | AGGCAAATGTAGCAGTAATCAAG | |
| CrmD5 | ATCTTGCACTGCGTTGTGAGGTC | ||||
| 3 | 50 | 2 | CrmD4 | GATTGTACTCCTGTCTTTATTGG | |
| CrmD2 | ATAACTTTCGTCATCGTTAAGTC | ||||
| crmE | 1 | 50 | 3 | K3R2 | CGGACGCGATATATTCCGACATGG |
| K3R4 | GGAGACAATAACTATTCGAGTCAC | ||||
| 2 | 55 | 3 | K3R3 | CCTTGGTGTCATAGTTGTAGAGGTCC | |
| K3R6 | GATGATTAAAAGTTAGGGAGGGGATG | ||||
| vCD30 | 1 | 50 | 2 | CD30-1 | GTTCTGGATACATGCACAAAG |
| CD30-2 | GGAGGATAATCATTTGCAAACG | ||||
| vSEMA | 1 | 50 | 2 | Sema-14 | TTTGTCGGATTGTCTATAGTAC |
| Sema-15 | TGCCGTGTATAAATCATAACC | ||||
| 2 | 50 | 2 | Sema-16 | TGATATAAACATATCAAAAGAAGG | |
| Sema-17 | TGGTATGTGGAACAACTTTTCC | ||||
| 3 | 50 | 2 | Sema-18 | AAAATTGGGAGGATATACAAAGC | |
| Sema-6 | CCCAAGCTTAATAGCGTTGCTGTAAAAATAATTCC | ||||
| IFN-γR | 1 | 55 | 3 | B8R-1 | TATATATTCGATCGCCATGGAC |
| B8R-4 | CGTATACAACAAGTAGATATCAC | ||||
| 2 | 55 | 3 | B8R-3 | CATATCAATTTGTACATCGAGC | |
| B8R-2 | TGAGGTTATGTTGATTATGCTACG | ||||
| VCP | 1 | 55 | 2.5 | VCP-1 | AAAGTAGCTGACATAAACCATTG |
| VCP-2 | ATTTGATCGGTGAATCTAAATCG | ||||
| 2 | 55 | 2.5 | VCP-3 | TTAACAGATTCACAAATAGGTGC | |
| VCP-4 | TTATTATTTGTACGATGTCCAGG | ||||
| VGF | 1 | 55 | 2 | VGF-3 | TCCCAATCTTGTTATAAACACAC |
| VGF-6 | TTTTTCTGAACGTTGATAGTCTAC | ||||
| 2 | 55 | 2 | VGF-7 | ATTGTTTACACGGTGTCTGTATC | |
| VGF-4 | TACAGATACATTGCATAGCATAGC |
a
Restriction sites added to the oligonucleotide sequence are shown in italics.