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. 2007 Nov 6;9(6):R77. doi: 10.1186/bcr1790

Figure 3.

Figure 3

Apoptotic and cell-cycle effects of pyranocoumarins on MDA-MB231 estrogen-independent breast cancer cells. (a) Cell-cycle distribution after 24 hours of treatment with decursin (D), decursinol angelate (DA) or decursinol (DL) at different exposure concentrations (10 μM, 20 μM, 50 μM) in complete medium. Cells prepared as in Figure 2 for flow cytometric analysis. Bar height represents average of duplicate flasks, variation less than 2%. (b) Apoptosis detected by the DNA fragmentation Death ELISA kit in MDA-MB231 cells after 24 hours of treatment with different exposure concentrations (50 μM, 100 μM, 200 μM) in complete medium. (c) Western blot analysis detection of cleaved poly(ADP-ribose) polymerase (c-PARP), estrogen receptor (ER)β and selected mitogen signaling and cell-cycle regulator proteins after 48 hours of treatment in complete medium.