Table 1.

Sequences of primers used in the real-time reverse transcription-polymerase chain reaction

Genes	Sequences of primers	Accession number	Target sites	Size (base pairs)
VEGF-A	F: 5'-TTTACTGCTGTACCTCCACCA-3'	M95200	125–423	298
	R: 5'-ATCTCTCCTATGTGCTGGCTTT-3'
VEGF-B	F: 5'-CCTGGAAGAACACAGCCAAT-3'	NM_011697	537–701	165
	R: 5'-GGAGTGGGATGGATGATGTC-3'
VEGF-C	F: 5'-GGGAAGAAGTTCCACCATCA-3'	NM_009506	1258–1392	135
	R: 5'-ATGTGGCCTTTTCCAATACG-3'
VEGF-D	F: 5'-GCTGTCACTGTTGCCCACTA-3'	NM_010216	1423–1611	207
	R: 5'-CCCTTCCTTTCTGAGTGCTG-3'
PLGF	F: 5'-GGGAAGAAGCAAGACATGGA-3'	NM_008827	1055–1261	189
	R: 5'-ATGTCCTGTCCCATCTCCAG-3'
β-actin	F: 5'-ACCCAGATCATGTTTGAGAC-3'	X03765	280–503	224
	R: 5'-GTCAGGATCTTCATGAGGTAGT-3'

F, forward primer; PLGF, placental growth factor; R, reverse primer; VEGF, vascular endothelial growth factor.