Table 1.
Gene-specific primer pairs used in real-time quantitative polymerase chain reaction
| Gene | Sense | Antisense | Amplicon length (base pair) | GenBank accession number |
| Aggrecan | 5'-ACA CCC CTA CCC TTG CTT CT-3' | 5'-AAA GTG TCC AAG GCA TCC AC-3' | 124 | NM_022190 |
| Ank | 5'-CAA GAG AGA CAG GGC CAA AG-3' | 5'-AAG GCA GCG AGA TAC AGG AA-3' | 173 | NM_053714 |
| L27 | 5'-TCC TGG CTG GAC GCT ACT C-3' | 5'-CCA CAG AGT ACC TTG TGG GC-3' | 227 | NM_022514 |
| PC-1 | 5'-TAT GCC CAA GAA AGG AAT GG-3' | 5'-GCA GCT GGT AAG CAC AAT GA-3' | 165 | NM_053535 |
| S29 | 5'-AAG ATG GGT CAC CAG CAG CTC TAC TG-3' | 5'-AGA CGC GGC AAG AGC GAG AA-3' | 67 | NM_012876 |
| TNAP | 5'-GAA CGT CAA TTA ACG GCT GA-3' | 5'-CAG ATG GGT GGG AAG AGG T-3' | 50 | NM_013059 |
| Type IA2 collagen | 5'-TTG ACC CTA ACC AAG GAT GC-3' | 5'-CAC CCC TTC TGC GTT GTA TT-3' | 197 | NM_053356 |
| Type II collagen | 5'-TCC CTC TGG TTC TGA TGG TC-3' | 5'-CTC TGT CTC CAG ATG CAC CA-3' | 161 | NM_012929 |
| Type X collagen | 5'-ATA TCC TGG GGA TCC AGG TC-3' | 5'-TGG GTC ACC CTT AGA TCC AG-3' | 241 | AJ131848 |
TNAP, tissue-nonspecific alkaline phosphatase.