Figure 2.

Biological activities of the serum opacity factor targeted to the phage by a Gram-positive signal sequence. (a) The serum opacity activity of the pSOF22 phagemid particles displaying the SOF22. A total of 10¹¹ phagemid particles were used per 200 μl assay. (b) Binding of the SOF22-displaying phagemid particles to human fibronectin detected by phage ELISA. A total of 10⁸ phagemid particles were used per assay, each carried out in a well of a 96-well plate. Samples: pSOF22 PP/d3 and pSOF22 PP/wt, phagemid particles displaying the SOF from S. pyogenes M22, generated using VCSM13d3 and VCSM13 helper phage, respectively; pDJ01 PP/wt, the vector phagemid particles, generated using the VCSM13 helper phage. BSA, TE, PBS, and BSA are buffer controls. Each data point is an average of three replicas; error bars represent standard deviation.