Figure 4.

Demonstration of the sarcosyl resistance selection step. (a) Free phagemid DNA (samples were loaded directly on a 0.8% agarose gel); (b) total DNA, the sum of the free DNA and DNA encapsulated in the phagemid particles (samples were heated at 70°C in 1.2% SDS for 10 minutes before loading, to disassemble the sarcosyl-resistant phagemid particles). Lanes: 1, library phagemid particles (PP) before incubation with sarcosyl; 2, after incubation with sarcosyl; 3, after incubation with sarcosyl and DNase I, followed by inactivation of DNase I).