Table 2.

Annotation of L. rhamnosus HN001 secretome ORFs

Category	Number of ORFs
Enzymes	20
Signal transduction components	1
Transport	22
Host/microbial interactions	3
Conserved, unknown function	28
Unique hypothetical proteins	7
Miscellaneous functions	2
Unclassified*	6
Total	89

*Short fragments of ORFs (encoding 27-57 amino acids) were fused to gIII; no hits above the threshold (e^-10) were detected using BlastP with automatic detection of short sequences. These ORFs were not classified as unique because the short length has prevented identifying potentially significant hits.