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. 2007 Oct 11;8(10):R220. doi: 10.1186/gb-2007-8-10-r220

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Copyright © 2007 Götz et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of transcript abundance quantified by microarray analysis and quantitative RT-PCR (qpcr). The changes in mRNA levels of six genes following UV irradiation were analyzed by quantitative RT-PCR to provide an independent confirmation of the microarray data. The log₂expression values for ssb (closed triangles), cdc6-2 (closed circles), tfb1 (closed squares), tfb2 (open squares), tfb3 (open circles) and dpoII (open triangles) obtained from microarray and RT-PCR analyses are plotted on the x and y axes, respectively. The data obtained from the two methods yield a linear fit with a slope of 0.93 and R-value of 0.91.