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. 1998 Apr 14;95(8):4188–4192. doi: 10.1073/pnas.95.8.4188

Figure 2.

Figure 2

Fractionation of 20S U5 and [U4/U6.U5] snRNPs under high salt conditions yields a Δ100-kDa U5 snRNP active in RNA unwinding. (a) The composition of proteins (Upper; for clarity, only high molecular weight proteins are shown) and snRNAs (Lower) after centrifugation in high salt (0.7 M KCl) glycerol gradients. (b) Immunodetection of the putative RNA helicases U5–200kD (Upper) and U5–100kD (Lower) from fractions displayed in a. (c) RNA unwinding activity of gradient fractions. An equivalent volume of each gradient fraction was assayed for RNA unwinding activity. Lane L, a mixture of 20S U5 and [U4/U6.U5] snRNPs before dissociation in 0.7 M KCl. The positions of proteins and snRNAs are indicated to the left.