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Percent change in ACh release in the hippocampus during spontaneous alternation testing. Note that ACh release increased significantly during testing only in 4-month-old controls and Ts65Dn mice. The magnitude of that increase declined with age in both genotypes. Ts65Dn mice had lower percent increase in ACh release than did same-age controls at 4 months (*Ps<0.05) but not at older ages. The numbers below bars indicate numbers of mice / group.
