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Kinetic data (±s.e.m., n=3) for the epimerization of UDP-GlcNAc by wild-type and mutant Bacillus anthracis UDP-GlcNAc 2-epimerase
| Enzyme | kcat (s−1) | KM (mM) | kcat/KM (s−1 mM−1) | n |
|---|---|---|---|---|
| Wild type | 7.9±0.3 | 2.2±0.2 | 3.6 | 1.4±0.1 |
| Arg210Ala | 0.021±0.001 | 20.9±2.2 | 0.001 | 1.15±0.07 |
| His242Ala | 0.22±0.10 | 10.4±1.0 | 0.021 | 1.47±0.15 |
| His44Gln | 4.0±0.2 | 7.3±0.8 | 0.55 | 1.21±0.08 |
| Gln70Ala | 2.06±0.07 | 11.1±0.8 | 0.19 | 1.24±0.06 |
| Gln43Ala | 1.20±0.07 | 17.2±1.6 | 0.070 | 1.77±0.23 |
| UDP-GlcNAc, UDP-N-acetylglucosamine. | ||||
