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Effect of rySec7d concentration on binding of GTP[γS] to ryARF2. ryARF2 (50 pmol) and 4 μM [35S]GTP[γS] were incubated with the indicated amount of rySec7d at 4°C for 3 h before radioassay of protein-bound [35S]GTP[γS]. rySec7d (2 μg) incubated alone bound no GTP[γS].
