Table 1.

Effect of BFA analogues on rySec7d-stimulated [³⁵S]GTP[γS] binding to ryARF2

BFA or analogue	% inhibition
Vehicle	4.34 ± 0.87
BFA	54.15 ± 4.31
B-17	10.95 ± 6.14
B-27	4.66 ± 1.67
B-36	7.10 ± 3.76

Fifty picomoles of ryARF2 and 4 μM [³⁵S]GTP[γS] were incubated with vehicle, BFA (9 μg), or analogues of BFA (B-17, B-27, or B-36) (9 μg of each) and 2 μg of rySec7d for 20 min at 30°C, as in Fig. 2A, before radioassay of protein-bound [³⁵S]GTP[γS]. All assays contained 1.2% CH₃OH. Data are means ± SEM of values from triplicate determinations. The experiment was replicated twice.