Figure 1.

Structure of the budding yeast centromere and organization of the CBF3 complex. (A) Schematic diagram of the budding yeast centromere element showing the location and scale of the CDE elements, as well as the consensus DNA composition in CDEIII. (B) Diagram of the four components of the CBF3 complex on DNA. (C) Schematic of the Cep3 sequence against Gal4 showing the locations of the conserved DNA-binding domain (yellow) and acidic patch (purple). The orange bar indicates the fragment described in this study. (D) Sequence alignment of members of the zinc-cluster domains from members of the Gal4-family of transcription factors against the amino-terminal of Cep3. The secondary structure elements are shown above the alignment, and totally conserved residues are shown in red.