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Analysis of purified single-headed L7/L12 dimer by gel electrophoresis. The FAST Gel system (Pharmacia) with 20% SDS polyacrylamide was used. Lanes: 1, mixture of oxidized and reduced Cys99; 2, single-headed dimer preparation; 3, NTF:Cys52; 4, single-headed dimer preparation after reduction with 2% β-mercaptoethanol.
