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Persistence of single-headed dimer after protein synthesis. Wild-type L7/L12 (lane 1), single-headed dimer (2 μg) (lane 2), and protein synthesis reaction mixture after 15 min of incubation containing 0.5 μg of single-headed dimer (lane 3) were separated by 16% SDS/PAGE, transferred to nitrocellulose and probed with monoclonal anti-NTF antibodies (20).
