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Activity of 70S ribosomes reconstituted from Po cores with L7/L12:Cys99
| L7/L12 variant added | Activity |
|---|---|
| Po | 3.5 |
| Po + L7/L12:Cys 99 - reduced | 12.5 |
| Po + L7/L12:Cys 99 - oxidized | 12.9 |
Results represent Phe/70S particle/15 min in poly[U]-directed polyphenylalanine synthesis (11) (average of three different experiments; SD was <10%). Six equivalents of each indicated protein were added to the Po cores.
