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. 1998 Apr 14;95(8):4235–4240. doi: 10.1073/pnas.95.8.4235

Figure 5.

Figure 5

Analysis of SL1 DNA, SL1 RNA, and ges-1 mRNA of Del U8-C12. The single nucleotide primer extension assay was performed by using 5′-GTT TCA ATG TTT ACC TCA TT-3′ for the analysis of the SL RNA gene and SL RNA and 5′-CAG AGC CAA GGA ATA TCC GCA TC-3′ for the analysis of the ges-1 message. Wild-type N2 worms contain only the wild-type SL1 RNA gene (lane 2), SL1 RNA (lane 4), and ges-1 message (lane 6). In rrs-1/+ worms transformed with the mutant gene, both wild-type and mutant SL1 RNA genes (lane 7 and 8) are detected. However, only wild-type SL1 RNA (lane 10) and ges-1 (lane 12) are detected in these worms. The absence of mutant SL1 RNA (lane 9) and mutant ges-1 (lane 11) indicates that the mutant SL1 RNA transcription or stability is eliminated. The arrow indicates the primer extended products.