Table 1.
Rescue of embryonic lethality of unc-76 rrs-1/unc-61 dpy-21 by transformation with SL1 RNA genes
| 100 μg/ml | 10 μg/ml | SL RNA present | Transfer to ges-1 | ||
|---|---|---|---|---|---|
| Wild type | 3/3 (1210) | 5/5 (1194) | + | + | |
| G22G23 to U22U23 | 0/5 (1541) | ND | +† | −† | |
| Class I | Del A27C28A29 | 1/1 (852) | 2/3 (706) | +* | ND |
| G16 to C16 | ND | 3/3 (608) | +* | ND | |
| Class II | A21 to C21 | 4/4 (1384) | 0/1 (211) | +* | ND |
| A21 to U21 | 8/8 (2812) | 0/3 (1369) | +* | ND | |
| Add C23A24G25 | 6/6 (3051) | 0/7 (1874) | +* | + | |
| Class III | Del U3-A7 | 0/3 (847) | ND | −† | −† |
| Del A6A7 | 0/9 (2590) | 0/3 (848) | −† | −† | |
| Del U8-C12 | 0/6 (1377) | ND | −† | −† | |
| Del C13-U17 | 0/3 (953) | ND | −† | −† | |
| Del G16-G20 | 0/8 (2365) | ND | ±† | −† | |
| A7U8U9A10C11 to C7A8C9C10A11 | 0/6 (2282) | ND | −† | −† |
Transforming DNA (100 μg/ml or 10 μg/ml) was used. The number of rescued lines over the number of transformed lines analyzed is shown.
*
The presence of mutant SL RNA or mutant SL on ges-1 mRNA was assayed using clones of rescued animals.
†
The assays were performed on mixed populations (wild-type and rrs-1 heterozygotes) of transgenic worms.
Numbers in parentheses designate the total number of progeny scored. Del, deletion; ND, not determined; Add, addition of nucleotides. Classes are described in text.