. 1998 Apr 14;95(8):4241–4246. doi: 10.1073/pnas.95.8.4241

Table 2.

Activation of the p_R promoter in various dnaA and rpoB mutants

Host	Activation of the p_R promoter
dnaA⁺ rpoB⁺	+++
dnaA46 rpoB⁺	−
dnaA⁺ rpoB902	+
dnaA46 rpoB902	++
dnaA204 rpoB⁺	−
dnaA204 rpoB902	−
dnaA204 rpoB903	++
dnaA508 rpoB⁺	−
dnaA508 rpoB902	−
dnaA508 rpoB904	+++

Activation of the p_R promoter was estimated on the basis of β-galactosidase activities measured in strains harboring fusions that consist of the lacZ gene under control of either p_R–“wild-type DnaA box” or p_R–“scrambled DnaA box” (see Fig. 2 for details). The value obtained with the wild-type fusion in the dnaA⁺ rpoB⁺ host (see Fig. 2) was considered as maximal (100%) activation, the value obtained with the fusion bearing the scrambled DnaA box was considered as no (0%) activation, and the values depicted in the table reflect these values (for each strain, activity of both fusions was measured and symbols presented in the table represent the activation of the wild-type fusion relative to the maximal activation). +++, 75–100% of maximal activation; ++, 50–75% of maximal activation; +, 25–50% of maximal activation; −, 0–25% of maximal activation. The experiments were performed at 30°C and 37°C, and very similar results were obtained at both temperatures.